Showing posts with label bacteria. Show all posts
Showing posts with label bacteria. Show all posts

Friday, May 17, 2013

What is the best method to know when bacteria is dying, or the best method to count bacteria in a petri dish?

Q. To clarify, if bacteria is in two petri dishes, and I add two different chemicals detrimental to the bacteria, what is the best way to tell which chemical is killing the bacteria fastest? Thanks!

A. I would suggest a method similar to that used in testing antimicrobial susceptibility. (1) Impregnate an known similar concetration of your chemical each on its own paper disk. (2) spreas your bacteria evenly on appropriate medium. (3) stamp the chemical disks on top of medium and incubate at 37 degrees for a day. (4) measure the zone of inhibition (area surrounding chemical disk with no bacterial growth). The larger the area, the more effective the chemical.
N.B. there may also be issues of chemical diffusion etc.


What happens to the bacteria after extracting the insulin or hormone?
Q. I know that we use bacteria for DNA recombinant. After we extract whatever we need from the bacteria, what happens to the bacteria? I really want to know.

A. The bacteria are usually killed in the process.
After all, it's not as if you could milk them.

In some cases, the desired end product can be sseparated from the bacteria without killing them, such as skimming it off. In that case a continuous production is possible. Otherwise, you make it in batches.


The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Q. The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Find the doubling period in minutes
Find the population after 115 minutes
When (in minutes) will thle population reach 15000?
4 hours ago - 4 days left to answer.

A. Long answer...
A = Pe^rt
A -represents the amount after a certain amount of time
P- represents the the amount at the start
r -represents the rate or speed and is always represented as a decimal
t- represents the amount of time in years, months, days, hours , minutes
e- is natural log = 2.718281828
see: http://www.algebralab.org/Word/Word.aspx?file=Algebra_InterestIII.xml

1st we find the rate or speed of growth represented by letter r
A = xe^rt
400= xe^r15
400/x =e^r15
As rule: ln e^x =x
Ln(400/x) = ln e^r15= r15
Rule: ln(a/b) = ln a - lnb
Ln 400 �ln x =15r
Ln 400 -15r =lnx
5.99146-15r =lnx
Have to do the same for the 1400 bacteria.
1400= xe^r30
1400/x =e^r30
Ln(1400/x) = ln e^r30= r30
Ln 1400 �ln x =30r
Ln 1400 -30r =lnx
7.242275 -30r =lnx
Set the ln x�s to equal
7.242275 -30r = 5.99146-15r
1.252763=15r
1.252763/15 = r =0.083517531
Faster way to find x:
400/x e^r15 =1400/xe^r30
e^r30/e^r15 =1400/400 (the x's cancel)
e^r15 = 3.5
ln e^r15 = ln 3.5
r15 =1.252762968
r =1.252762968/15 =0.083517531

Now we can find initial amount x.
400= xe^(0.083517531)(15)
400 =x e^1.252763
e- is natural log = 2.718281828 , plug # in calculator and hit inverse ln works faster
e^1.252763 =3.5
400=x(3.5)
400/3.5 = x = 114.286 =P

The values for P and R never change.

What was the initial size? P =114.286 is initial amount

Find the doubling period in minutes. For doubling
Let left side (A/P) equal 2 and then find time.
2 = e^(0.083517531)t
Ln 2 =(0.083517531)t
0.69314718/0.083517531 = t = 8.3 min


Find the population after 115 minutes. Let t =115 and find A = 1,695,041

When (in minutes) will the population reach 15000? Let A =15000 and find t = 58.4 minutes
A T e^rt
400153.5
228.57148.292
1,00025.978.75
1,4003012.25
15,00058.39131.25
210,087.5901838.266
1,695,04111514831.61
5,932,64413051910.64
31,526,254150275854.7


What the three main and nastiest group of bacteria ruling earth presently?
Q. What are the main groups of bacteria ruling earth now?

There are three main groups of bacteria ruling earth from the Arabic world: The moon bacteria, the sun bacteria, and the stars bacteria, which call themselves 666.

As their ability is to mimic and replicate they have replaced the original worshipers of the sun and keep them enslaved. These Asian vermins have become white, black, yellow, and all species that they can replicate and so are the insects that are part of bacteria�s retinue.

The moon bacteria are worshipers of the moon. They live mostly in darkness or shade and the insects serving them are night insects and if they are there during the day, they keep the sun at a lower level of heat using the clouds.

The sun bacteria are not really from flame planet, but use the light that pierces the clouds to glorify themselves as the sun. In fact there is no bacteria that can resist flame.

The stars bacteria are the different branches of bacteria that are part or under the leadership of bacteria. They are replication of the main group. That is why when one kingdom does something, all other kingdoms follow.

These three main groups are the nastiest bacteria ruling earth. Bacteria keep surfacing the planet as the scientist bacteria are digging the earth to bring them out.

These bacteria are living entities that feed on decay. They lived in people before, those that were buried. They are waiting to be called to service because their hosts die. They take turn to rule. Those that live in king and kill their rulers have highest privileges. The privileges go by their faces on money and other things in the kingdom they rule. That is why only dead people are on money. As such, the vermins that ate these poor people made rich by bacteria are rewarded with their face on money, celebration of their prowess every year in the form of memorial and other nastiness that they did.

Their willing temples, the priests, and the rich, allowed them to do so as long as they continue to be in power.


Yah is a bacterial moon god. As all bacterial gods, they are bloodless and heartless. They are flies, ticks, lice, mites, roaches, and all types of seen and unseen bacteria. Bah, cah, tah, nah, and all the 26 letters of all alphabets in any languages are bacteria's names.

A. LIFE CONSUMES LIFE
~~~~~~~~~~~~
Life consumes Life
Take it for what it�s worth
Birth begs to be fed
Earth ate the dead
No matter what the spread
Iron Oxide rust red
�Life consumes Life� I said
Phage out numbers all of life combined
Bores into bacteria
Consumes until it dies
A Murder of crows to pick out the eyes
An eating frenzy never satisfied
Life is a banquet
A cannibalistic feast
For everything is the fodder
From the great whale to the hungered yeast





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Tuesday, May 7, 2013

How can energy in bacteria cells be released if they have no mitochondria?

Q. Prokaryotic bacteria cells don't have mitochondria so how is energy released for the cells to use them?

A. Since mitochondria likely evolved from bacteria by endosymbiosis, we can figure out how bacteria can do the things that mitochondria do.

First, the reactions of glycolysis take place in the cytoplasm of the bacteria, as do the reactions of the citric acid cycle. The proteins of the electron transport chain are located in the plasma membranes of the bacteria. The protein ATP synthase is also found in the plasma membrane of bacteria.

You can think of the matrix of the mitochondrion as comparable to the bacterial cytoplasm. The inner mitochondrial membrane is equivalent to the plasma membrane of the bacteria.


What specific strains of bacteria are used in wine production and what are the used for?
Q. I need bacteria only (no other types of organisms) and the specific strains of bacteria and description of them or any sources where I can find the information. Thanks.

A. Wine is primarily made using yeasts such as Saccharomyces cerevisiae, via alcoholic fermentation. The pyruvate decarboxylase enzyme used by these yeasts to produce ethanol is rare in bacteria, however there are a few bacterial organisms than can carry out alcoholic fermentation.

Zymomonas mobilis is one of the rare species that does possess the pyruvate decarboxylase, and would be able to produce ethanol. It can be isoalted from the Mexcian alcoholic beverage "pulque".

Sarcina ventriculi and Ervinia amylovora are other representative organisms that can produce ethanol from fermentation.

Many lactic acid bacteria, enterobacteria, and clostridia can produce considerable ethanol without the pyruvate decarboxylase. Instead, they convert:
pyruate -> acetyl-CoA -> acetaldehyde -> ethanol, alcohol dehydrogenase.

It has been of high interest to the alcohol industry to find organisms that can produce ethanol under high temperature, to prevent contamination of unwanted organisms. Some bacterial species capable of such alcoholic fermentation at high temperatures include: Clostridium thermohydrosulfuricum, Thermoanaerobium brockii, and Thermoanaerobacter ethanolicus. Note that these organisms lack pyruvate decarboxylase and produce considerable acetate and lactate in addition to ethanol.

Hope this helps!


How does these different types of bacteria play a role in the nitrogen cycle?
Q. What roles do these bacteria play in the nitrogen cycle?
1. Free-living soil bacteria
2. Root nodule bacteria
3. Nitrifying bacteria
4. Denitrifying bacteria

A. Free living soil bacteria does ammonification.root nodule bacteria fix nitrogen.nitrogen fixing bacteria oxidize ammonia to nitrite and nitrite to nitrate.dinitrifying bacteria reduced ammonia to atmospheqic nitrogen


What is the best method to know when bacteria is dying, or the best method to count bacteria in a petri dish?
Q. To clarify, if bacteria is in two petri dishes, and I add two different chemicals detrimental to the bacteria, what is the best way to tell which chemical is killing the bacteria fastest? Thanks!

A. I would suggest a method similar to that used in testing antimicrobial susceptibility. (1) Impregnate an known similar concetration of your chemical each on its own paper disk. (2) spreas your bacteria evenly on appropriate medium. (3) stamp the chemical disks on top of medium and incubate at 37 degrees for a day. (4) measure the zone of inhibition (area surrounding chemical disk with no bacterial growth). The larger the area, the more effective the chemical.
N.B. there may also be issues of chemical diffusion etc.





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Thursday, April 25, 2013

What is the best method to know when bacteria is dying, or the best method to count bacteria in a petri dish?

Q. To clarify, if bacteria is in two petri dishes, and I add two different chemicals detrimental to the bacteria, what is the best way to tell which chemical is killing the bacteria fastest? Thanks!

A. I would suggest a method similar to that used in testing antimicrobial susceptibility. (1) Impregnate an known similar concetration of your chemical each on its own paper disk. (2) spreas your bacteria evenly on appropriate medium. (3) stamp the chemical disks on top of medium and incubate at 37 degrees for a day. (4) measure the zone of inhibition (area surrounding chemical disk with no bacterial growth). The larger the area, the more effective the chemical.
N.B. there may also be issues of chemical diffusion etc.


What happens to the bacteria after extracting the insulin or hormone?
Q. I know that we use bacteria for DNA recombinant. After we extract whatever we need from the bacteria, what happens to the bacteria? I really want to know.

A. The bacteria are usually killed in the process.
After all, it's not as if you could milk them.

In some cases, the desired end product can be sseparated from the bacteria without killing them, such as skimming it off. In that case a continuous production is possible. Otherwise, you make it in batches.


The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Q. The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Find the doubling period in minutes
Find the population after 115 minutes
When (in minutes) will thle population reach 15000?
4 hours ago - 4 days left to answer.

A. Long answer...
A = Pe^rt
A -represents the amount after a certain amount of time
P- represents the the amount at the start
r -represents the rate or speed and is always represented as a decimal
t- represents the amount of time in years, months, days, hours , minutes
e- is natural log = 2.718281828
see: http://www.algebralab.org/Word/Word.aspx?file=Algebra_InterestIII.xml

1st we find the rate or speed of growth represented by letter r
A = xe^rt
400= xe^r15
400/x =e^r15
As rule: ln e^x =x
Ln(400/x) = ln e^r15= r15
Rule: ln(a/b) = ln a - lnb
Ln 400 �ln x =15r
Ln 400 -15r =lnx
5.99146-15r =lnx
Have to do the same for the 1400 bacteria.
1400= xe^r30
1400/x =e^r30
Ln(1400/x) = ln e^r30= r30
Ln 1400 �ln x =30r
Ln 1400 -30r =lnx
7.242275 -30r =lnx
Set the ln x�s to equal
7.242275 -30r = 5.99146-15r
1.252763=15r
1.252763/15 = r =0.083517531
Faster way to find x:
400/x e^r15 =1400/xe^r30
e^r30/e^r15 =1400/400 (the x's cancel)
e^r15 = 3.5
ln e^r15 = ln 3.5
r15 =1.252762968
r =1.252762968/15 =0.083517531

Now we can find initial amount x.
400= xe^(0.083517531)(15)
400 =x e^1.252763
e- is natural log = 2.718281828 , plug # in calculator and hit inverse ln works faster
e^1.252763 =3.5
400=x(3.5)
400/3.5 = x = 114.286 =P

The values for P and R never change.

What was the initial size? P =114.286 is initial amount

Find the doubling period in minutes. For doubling
Let left side (A/P) equal 2 and then find time.
2 = e^(0.083517531)t
Ln 2 =(0.083517531)t
0.69314718/0.083517531 = t = 8.3 min


Find the population after 115 minutes. Let t =115 and find A = 1,695,041

When (in minutes) will the population reach 15000? Let A =15000 and find t = 58.4 minutes
A T e^rt
400153.5
228.57148.292
1,00025.978.75
1,4003012.25
15,00058.39131.25
210,087.5901838.266
1,695,04111514831.61
5,932,64413051910.64
31,526,254150275854.7


What the three main and nastiest group of bacteria ruling earth presently?
Q. What are the main groups of bacteria ruling earth now?

There are three main groups of bacteria ruling earth from the Arabic world: The moon bacteria, the sun bacteria, and the stars bacteria, which call themselves 666.

As their ability is to mimic and replicate they have replaced the original worshipers of the sun and keep them enslaved. These Asian vermins have become white, black, yellow, and all species that they can replicate and so are the insects that are part of bacteria�s retinue.

The moon bacteria are worshipers of the moon. They live mostly in darkness or shade and the insects serving them are night insects and if they are there during the day, they keep the sun at a lower level of heat using the clouds.

The sun bacteria are not really from flame planet, but use the light that pierces the clouds to glorify themselves as the sun. In fact there is no bacteria that can resist flame.

The stars bacteria are the different branches of bacteria that are part or under the leadership of bacteria. They are replication of the main group. That is why when one kingdom does something, all other kingdoms follow.

These three main groups are the nastiest bacteria ruling earth. Bacteria keep surfacing the planet as the scientist bacteria are digging the earth to bring them out.

These bacteria are living entities that feed on decay. They lived in people before, those that were buried. They are waiting to be called to service because their hosts die. They take turn to rule. Those that live in king and kill their rulers have highest privileges. The privileges go by their faces on money and other things in the kingdom they rule. That is why only dead people are on money. As such, the vermins that ate these poor people made rich by bacteria are rewarded with their face on money, celebration of their prowess every year in the form of memorial and other nastiness that they did.

Their willing temples, the priests, and the rich, allowed them to do so as long as they continue to be in power.


Yah is a bacterial moon god. As all bacterial gods, they are bloodless and heartless. They are flies, ticks, lice, mites, roaches, and all types of seen and unseen bacteria. Bah, cah, tah, nah, and all the 26 letters of all alphabets in any languages are bacteria's names.

A. LIFE CONSUMES LIFE
~~~~~~~~~~~~
Life consumes Life
Take it for what it�s worth
Birth begs to be fed
Earth ate the dead
No matter what the spread
Iron Oxide rust red
�Life consumes Life� I said
Phage out numbers all of life combined
Bores into bacteria
Consumes until it dies
A Murder of crows to pick out the eyes
An eating frenzy never satisfied
Life is a banquet
A cannibalistic feast
For everything is the fodder
From the great whale to the hungered yeast





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Saturday, April 20, 2013

How much smaller is bacteria than dust or human hair strand that is the smallest human eye can see?

Q. Also is true that all types of bacteria cannot see ? If all types of bacteria cannot see how do they sense the world smell or chemicals?

The bacteria have no light rods or cones?

A. Bacteria are smaller than most eukaryotic cells, such as body cells. Viruses are so small they can infect bacteria.
Not bacteria can see. Some Protista have light sensitive patches to guide them to food, but they can't "see". Bacteria instead float around. They use chemicals to communicate with the world around them.


What important roles do bacteria play in the deep ocean? What would happen if they werenʼt there?
Q. What important roles do bacteria play in the deep ocean? What would happen if
they werenʼt there?

A. Bacteria serve to rid the ocean floor of dead organisms by decomposing them. If they weren't there, the ocean floor would accumulate too much biomass.


How long does it take for good bacteria to form in a fish tank.And does the good bacteria break down the poop.?
Q. Its self.I know that the good bacteria feeds on ammonia but does it break down the poop it self.And how long does it take the good bacteria to form in the plastic case of the filter.I hear the term the filter media what does that mean.And how long can i go before i have to clean it.

A. Pooh will rot away causing amonia.. good bacteria will then eat the amonia converting it into nitrite..

A differnt type of bacteria needs to form eating the nitrite turning it into nitrate...

Nitrate is removed from he tank by frequent water changes (live plants and algae will also eat the nitrate)

The colonies of bacterias can take up to 6 months to form.. they form in the filter, gravel, and on any ornaments.. So it is best to only add a couple of hardy fish (zebra danios) for this period or read up on a fishless cycle..

Filter Media - is the sponglike inserts you put into a filter. There are variations on this depending on your filter.. After the intial 6 month period described above it is best to rinse the media in old tank water to maintain bacteria colony.. do this monthly..
And if it too blocked and need replacing only ever change half of the media at a time and ensure you rinse in old tank water

You use tank water you have poured into a seperate container because the chlorine is too high in our tap water and this will kill the fish.


What temperature is good bacteria killed?
Q. I always hear bad bacteria is killed at 145 degrees for a certain amount of time, but what about good bacteria. Is it killed in the same range or at higher temperatures? I hear that low temperature pasterization of milk kills off the bad bacteria and preserves some of the good bacteria and it's heated at 145 degrees for 30 minutes. Is this true or are they giving false statements to sell this milk?

A. Unless the bacterium has an endospore which is resistant to heat and chemicals, the thermal death time is the same for most species of bacteria, good or bad. Pasteurization of milk is intended to kill pathogenic bacteria typically found in milk. The standards for the quality of milk is set by state and federal agencies. Those bacteria found in pasteurized milk are considered thermoduric (tolerate the pasteurization temperature) and psychrophilic (can grow at refrigeration temperatures) and will eventually cause the milk to spoil. With ultra-pasteurization, milk does not have to be refrigerated.





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Thursday, April 11, 2013

How long does it take for good bacteria to form in a fish tank.And does the good bacteria break down the poop.?

Q. Its self.I know that the good bacteria feeds on ammonia but does it break down the poop it self.And how long does it take the good bacteria to form in the plastic case of the filter.I hear the term the filter media what does that mean.And how long can i go before i have to clean it.

A. Pooh will rot away causing amonia.. good bacteria will then eat the amonia converting it into nitrite..

A differnt type of bacteria needs to form eating the nitrite turning it into nitrate...

Nitrate is removed from he tank by frequent water changes (live plants and algae will also eat the nitrate)

The colonies of bacterias can take up to 6 months to form.. they form in the filter, gravel, and on any ornaments.. So it is best to only add a couple of hardy fish (zebra danios) for this period or read up on a fishless cycle..

Filter Media - is the sponglike inserts you put into a filter. There are variations on this depending on your filter.. After the intial 6 month period described above it is best to rinse the media in old tank water to maintain bacteria colony.. do this monthly..
And if it too blocked and need replacing only ever change half of the media at a time and ensure you rinse in old tank water

You use tank water you have poured into a seperate container because the chlorine is too high in our tap water and this will kill the fish.


What beneficial purpose does bacteria on our skin serve?
Q. I know we're teeming with bacteria on our skin. Like the bacteria in our intestines, I'm assuming the skin bacteria serve a specific beneficial purpose. What is the benefit and would you please be as specific as possible? Thank you very much!

A. for these normal flora bacteria, as they occupy the places, many pathogenic bacteria cant multiply in skin, the normal flora bacteria dont let them develop due to competition


How do you make bacteria grow faster?
Q. My little sister is doing a bacteria experiment for her science fair. She completed the steps that a instruction video told us and require 3-7 days to grow. Today is the 2nd day and the science fair is tommorrow night. Is their anyway we can get the bacteria to grow faster?

We poured agar in pitri dishes and then swabbed surfaces in order to collect it.
We collected the bacteria from a elementary school. So bathroom handles, folders, pencils, door knobs, etc.
We originally kept them in a drawer in the kitchen with a towel over them.

A. Bacteria requires a protein source,moisture and an ideal temperature of about 32 to 39 degrees Celsius Bacteria also multiply about every 20 minutes.


is it possible for bacteria inside an organism to survive outside the organism?
Q. I am planning to research a bacteria in mealworms that allows them to digest polystyrene (Styrofoam). If I take the bacteria out of the mealworm, will they die?

A. From what I learned, yes, the bacteria living inside a cell does not usually survive long unless you mimic the exact environment of the inside of the cell. Some cells can survive outside of their natural habitats but not for long usually.





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Monday, April 8, 2013

What is the best method to know when bacteria is dying, or the best method to count bacteria in a petri dish?

Q. To clarify, if bacteria is in two petri dishes, and I add two different chemicals detrimental to the bacteria, what is the best way to tell which chemical is killing the bacteria fastest? Thanks!

A. I would suggest a method similar to that used in testing antimicrobial susceptibility. (1) Impregnate an known similar concetration of your chemical each on its own paper disk. (2) spreas your bacteria evenly on appropriate medium. (3) stamp the chemical disks on top of medium and incubate at 37 degrees for a day. (4) measure the zone of inhibition (area surrounding chemical disk with no bacterial growth). The larger the area, the more effective the chemical.
N.B. there may also be issues of chemical diffusion etc.


What happens to the bacteria after extracting the insulin or hormone?
Q. I know that we use bacteria for DNA recombinant. After we extract whatever we need from the bacteria, what happens to the bacteria? I really want to know.

A. The bacteria are usually killed in the process.
After all, it's not as if you could milk them.

In some cases, the desired end product can be sseparated from the bacteria without killing them, such as skimming it off. In that case a continuous production is possible. Otherwise, you make it in batches.


The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Q. The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Find the doubling period in minutes
Find the population after 115 minutes
When (in minutes) will thle population reach 15000?
4 hours ago - 4 days left to answer.

A. Long answer...
A = Pe^rt
A -represents the amount after a certain amount of time
P- represents the the amount at the start
r -represents the rate or speed and is always represented as a decimal
t- represents the amount of time in years, months, days, hours , minutes
e- is natural log = 2.718281828
see: http://www.algebralab.org/Word/Word.aspx?file=Algebra_InterestIII.xml

1st we find the rate or speed of growth represented by letter r
A = xe^rt
400= xe^r15
400/x =e^r15
As rule: ln e^x =x
Ln(400/x) = ln e^r15= r15
Rule: ln(a/b) = ln a - lnb
Ln 400 �ln x =15r
Ln 400 -15r =lnx
5.99146-15r =lnx
Have to do the same for the 1400 bacteria.
1400= xe^r30
1400/x =e^r30
Ln(1400/x) = ln e^r30= r30
Ln 1400 �ln x =30r
Ln 1400 -30r =lnx
7.242275 -30r =lnx
Set the ln x�s to equal
7.242275 -30r = 5.99146-15r
1.252763=15r
1.252763/15 = r =0.083517531
Faster way to find x:
400/x e^r15 =1400/xe^r30
e^r30/e^r15 =1400/400 (the x's cancel)
e^r15 = 3.5
ln e^r15 = ln 3.5
r15 =1.252762968
r =1.252762968/15 =0.083517531

Now we can find initial amount x.
400= xe^(0.083517531)(15)
400 =x e^1.252763
e- is natural log = 2.718281828 , plug # in calculator and hit inverse ln works faster
e^1.252763 =3.5
400=x(3.5)
400/3.5 = x = 114.286 =P

The values for P and R never change.

What was the initial size? P =114.286 is initial amount

Find the doubling period in minutes. For doubling
Let left side (A/P) equal 2 and then find time.
2 = e^(0.083517531)t
Ln 2 =(0.083517531)t
0.69314718/0.083517531 = t = 8.3 min


Find the population after 115 minutes. Let t =115 and find A = 1,695,041

When (in minutes) will the population reach 15000? Let A =15000 and find t = 58.4 minutes
A T e^rt
400153.5
228.57148.292
1,00025.978.75
1,4003012.25
15,00058.39131.25
210,087.5901838.266
1,695,04111514831.61
5,932,64413051910.64
31,526,254150275854.7


What the three main and nastiest group of bacteria ruling earth presently?
Q. What are the main groups of bacteria ruling earth now?

There are three main groups of bacteria ruling earth from the Arabic world: The moon bacteria, the sun bacteria, and the stars bacteria, which call themselves 666.

As their ability is to mimic and replicate they have replaced the original worshipers of the sun and keep them enslaved. These Asian vermins have become white, black, yellow, and all species that they can replicate and so are the insects that are part of bacteria�s retinue.

The moon bacteria are worshipers of the moon. They live mostly in darkness or shade and the insects serving them are night insects and if they are there during the day, they keep the sun at a lower level of heat using the clouds.

The sun bacteria are not really from flame planet, but use the light that pierces the clouds to glorify themselves as the sun. In fact there is no bacteria that can resist flame.

The stars bacteria are the different branches of bacteria that are part or under the leadership of bacteria. They are replication of the main group. That is why when one kingdom does something, all other kingdoms follow.

These three main groups are the nastiest bacteria ruling earth. Bacteria keep surfacing the planet as the scientist bacteria are digging the earth to bring them out.

These bacteria are living entities that feed on decay. They lived in people before, those that were buried. They are waiting to be called to service because their hosts die. They take turn to rule. Those that live in king and kill their rulers have highest privileges. The privileges go by their faces on money and other things in the kingdom they rule. That is why only dead people are on money. As such, the vermins that ate these poor people made rich by bacteria are rewarded with their face on money, celebration of their prowess every year in the form of memorial and other nastiness that they did.

Their willing temples, the priests, and the rich, allowed them to do so as long as they continue to be in power.


Yah is a bacterial moon god. As all bacterial gods, they are bloodless and heartless. They are flies, ticks, lice, mites, roaches, and all types of seen and unseen bacteria. Bah, cah, tah, nah, and all the 26 letters of all alphabets in any languages are bacteria's names.

A. LIFE CONSUMES LIFE
~~~~~~~~~~~~
Life consumes Life
Take it for what it�s worth
Birth begs to be fed
Earth ate the dead
No matter what the spread
Iron Oxide rust red
�Life consumes Life� I said
Phage out numbers all of life combined
Bores into bacteria
Consumes until it dies
A Murder of crows to pick out the eyes
An eating frenzy never satisfied
Life is a banquet
A cannibalistic feast
For everything is the fodder
From the great whale to the hungered yeast





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Monday, April 1, 2013

How can energy in bacteria cells be released if they have no mitochondria?

Q. Prokaryotic bacteria cells don't have mitochondria so how is energy released for the cells to use them?

A. Since mitochondria likely evolved from bacteria by endosymbiosis, we can figure out how bacteria can do the things that mitochondria do.

First, the reactions of glycolysis take place in the cytoplasm of the bacteria, as do the reactions of the citric acid cycle. The proteins of the electron transport chain are located in the plasma membranes of the bacteria. The protein ATP synthase is also found in the plasma membrane of bacteria.

You can think of the matrix of the mitochondrion as comparable to the bacterial cytoplasm. The inner mitochondrial membrane is equivalent to the plasma membrane of the bacteria.


What specific strains of bacteria are used in wine production and what are the used for?
Q. I need bacteria only (no other types of organisms) and the specific strains of bacteria and description of them or any sources where I can find the information. Thanks.

A. Wine is primarily made using yeasts such as Saccharomyces cerevisiae, via alcoholic fermentation. The pyruvate decarboxylase enzyme used by these yeasts to produce ethanol is rare in bacteria, however there are a few bacterial organisms than can carry out alcoholic fermentation.

Zymomonas mobilis is one of the rare species that does possess the pyruvate decarboxylase, and would be able to produce ethanol. It can be isoalted from the Mexcian alcoholic beverage "pulque".

Sarcina ventriculi and Ervinia amylovora are other representative organisms that can produce ethanol from fermentation.

Many lactic acid bacteria, enterobacteria, and clostridia can produce considerable ethanol without the pyruvate decarboxylase. Instead, they convert:
pyruate -> acetyl-CoA -> acetaldehyde -> ethanol, alcohol dehydrogenase.

It has been of high interest to the alcohol industry to find organisms that can produce ethanol under high temperature, to prevent contamination of unwanted organisms. Some bacterial species capable of such alcoholic fermentation at high temperatures include: Clostridium thermohydrosulfuricum, Thermoanaerobium brockii, and Thermoanaerobacter ethanolicus. Note that these organisms lack pyruvate decarboxylase and produce considerable acetate and lactate in addition to ethanol.

Hope this helps!


How does these different types of bacteria play a role in the nitrogen cycle?
Q. What roles do these bacteria play in the nitrogen cycle?
1. Free-living soil bacteria
2. Root nodule bacteria
3. Nitrifying bacteria
4. Denitrifying bacteria

A. Free living soil bacteria does ammonification.root nodule bacteria fix nitrogen.nitrogen fixing bacteria oxidize ammonia to nitrite and nitrite to nitrate.dinitrifying bacteria reduced ammonia to atmospheqic nitrogen


How do the beneficial bacteria in your body survive when we take antibiotics?
Q. We have some good bacteria, like in our intestines, that we need. But antibiotics kill all types of bacteria. How do the good bacteria survive?

A. The majority of them do. The intestines host an absolutely enormous population of bacteria, primarily in the large intestine and below. You would have to take very large doses of antibiotics for quite some time to kill everything in there.

Even then, the overwhelming majority of bacteria in the human intestines are members of a genus called Bacteroides, which are -very- resistant to most conventional antibiotics, and produce extended-spectrum beta-lactamase enzymes, which locally inactivates a wide range of compounds.

The basic gist of it is that these bacteria are present in large numbers, are not completely killed off, and have a population doubling time of usually around an hour and change, so if they're killed, they grow back quickly.

It's also important to remember that not all antibiotics affect all bacteria, and that those 'good' bacteria are a very frequent source of opportunistic infections, UTIs and the like when they get into places they're not supposed to be.





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Saturday, March 23, 2013

The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?

Q. The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Find the doubling period in minutes
Find the population after 115 minutes
When (in minutes) will thle population reach 15000?
4 hours ago - 4 days left to answer.

A. Long answer...
A = Pe^rt
A -represents the amount after a certain amount of time
P- represents the the amount at the start
r -represents the rate or speed and is always represented as a decimal
t- represents the amount of time in years, months, days, hours , minutes
e- is natural log = 2.718281828
see: http://www.algebralab.org/Word/Word.aspx?file=Algebra_InterestIII.xml

1st we find the rate or speed of growth represented by letter r
A = xe^rt
400= xe^r15
400/x =e^r15
As rule: ln e^x =x
Ln(400/x) = ln e^r15= r15
Rule: ln(a/b) = ln a - lnb
Ln 400 �ln x =15r
Ln 400 -15r =lnx
5.99146-15r =lnx
Have to do the same for the 1400 bacteria.
1400= xe^r30
1400/x =e^r30
Ln(1400/x) = ln e^r30= r30
Ln 1400 �ln x =30r
Ln 1400 -30r =lnx
7.242275 -30r =lnx
Set the ln x�s to equal
7.242275 -30r = 5.99146-15r
1.252763=15r
1.252763/15 = r =0.083517531
Faster way to find x:
400/x e^r15 =1400/xe^r30
e^r30/e^r15 =1400/400 (the x's cancel)
e^r15 = 3.5
ln e^r15 = ln 3.5
r15 =1.252762968
r =1.252762968/15 =0.083517531

Now we can find initial amount x.
400= xe^(0.083517531)(15)
400 =x e^1.252763
e- is natural log = 2.718281828 , plug # in calculator and hit inverse ln works faster
e^1.252763 =3.5
400=x(3.5)
400/3.5 = x = 114.286 =P

The values for P and R never change.

What was the initial size? P =114.286 is initial amount

Find the doubling period in minutes. For doubling
Let left side (A/P) equal 2 and then find time.
2 = e^(0.083517531)t
Ln 2 =(0.083517531)t
0.69314718/0.083517531 = t = 8.3 min


Find the population after 115 minutes. Let t =115 and find A = 1,695,041

When (in minutes) will the population reach 15000? Let A =15000 and find t = 58.4 minutes
A T e^rt
400153.5
228.57148.292
1,00025.978.75
1,4003012.25
15,00058.39131.25
210,087.5901838.266
1,695,04111514831.61
5,932,64413051910.64
31,526,254150275854.7


What the three main and nastiest group of bacteria ruling earth presently?
Q. What are the main groups of bacteria ruling earth now?

There are three main groups of bacteria ruling earth from the Arabic world: The moon bacteria, the sun bacteria, and the stars bacteria, which call themselves 666.

As their ability is to mimic and replicate they have replaced the original worshipers of the sun and keep them enslaved. These Asian vermins have become white, black, yellow, and all species that they can replicate and so are the insects that are part of bacteria�s retinue.

The moon bacteria are worshipers of the moon. They live mostly in darkness or shade and the insects serving them are night insects and if they are there during the day, they keep the sun at a lower level of heat using the clouds.

The sun bacteria are not really from flame planet, but use the light that pierces the clouds to glorify themselves as the sun. In fact there is no bacteria that can resist flame.

The stars bacteria are the different branches of bacteria that are part or under the leadership of bacteria. They are replication of the main group. That is why when one kingdom does something, all other kingdoms follow.

These three main groups are the nastiest bacteria ruling earth. Bacteria keep surfacing the planet as the scientist bacteria are digging the earth to bring them out.

These bacteria are living entities that feed on decay. They lived in people before, those that were buried. They are waiting to be called to service because their hosts die. They take turn to rule. Those that live in king and kill their rulers have highest privileges. The privileges go by their faces on money and other things in the kingdom they rule. That is why only dead people are on money. As such, the vermins that ate these poor people made rich by bacteria are rewarded with their face on money, celebration of their prowess every year in the form of memorial and other nastiness that they did.

Their willing temples, the priests, and the rich, allowed them to do so as long as they continue to be in power.


Yah is a bacterial moon god. As all bacterial gods, they are bloodless and heartless. They are flies, ticks, lice, mites, roaches, and all types of seen and unseen bacteria. Bah, cah, tah, nah, and all the 26 letters of all alphabets in any languages are bacteria's names.

A. LIFE CONSUMES LIFE
~~~~~~~~~~~~
Life consumes Life
Take it for what it�s worth
Birth begs to be fed
Earth ate the dead
No matter what the spread
Iron Oxide rust red
�Life consumes Life� I said
Phage out numbers all of life combined
Bores into bacteria
Consumes until it dies
A Murder of crows to pick out the eyes
An eating frenzy never satisfied
Life is a banquet
A cannibalistic feast
For everything is the fodder
From the great whale to the hungered yeast


How much smaller is bacteria than dust or human hair strand that is the smallest human eye can see?
Q. Also is true that all types of bacteria cannot see ? If all types of bacteria cannot see how do they sense the world smell or chemicals?

The bacteria have no light rods or cones?

A. Bacteria are smaller than most eukaryotic cells, such as body cells. Viruses are so small they can infect bacteria.
Not bacteria can see. Some Protista have light sensitive patches to guide them to food, but they can't "see". Bacteria instead float around. They use chemicals to communicate with the world around them.


What important roles do bacteria play in the deep ocean? What would happen if they werenʼt there?
Q. What important roles do bacteria play in the deep ocean? What would happen if
they werenʼt there?

A. Bacteria serve to rid the ocean floor of dead organisms by decomposing them. If they weren't there, the ocean floor would accumulate too much biomass.





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What happens to the bacteria after extracting the insulin or hormone?

Q. I know that we use bacteria for DNA recombinant. After we extract whatever we need from the bacteria, what happens to the bacteria? I really want to know.

A. The bacteria are usually killed in the process.
After all, it's not as if you could milk them.

In some cases, the desired end product can be sseparated from the bacteria without killing them, such as skimming it off. In that case a continuous production is possible. Otherwise, you make it in batches.


The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Q. The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Find the doubling period in minutes
Find the population after 115 minutes
When (in minutes) will thle population reach 15000?
4 hours ago - 4 days left to answer.

A. Long answer...
A = Pe^rt
A -represents the amount after a certain amount of time
P- represents the the amount at the start
r -represents the rate or speed and is always represented as a decimal
t- represents the amount of time in years, months, days, hours , minutes
e- is natural log = 2.718281828
see: http://www.algebralab.org/Word/Word.aspx?file=Algebra_InterestIII.xml

1st we find the rate or speed of growth represented by letter r
A = xe^rt
400= xe^r15
400/x =e^r15
As rule: ln e^x =x
Ln(400/x) = ln e^r15= r15
Rule: ln(a/b) = ln a - lnb
Ln 400 �ln x =15r
Ln 400 -15r =lnx
5.99146-15r =lnx
Have to do the same for the 1400 bacteria.
1400= xe^r30
1400/x =e^r30
Ln(1400/x) = ln e^r30= r30
Ln 1400 �ln x =30r
Ln 1400 -30r =lnx
7.242275 -30r =lnx
Set the ln x�s to equal
7.242275 -30r = 5.99146-15r
1.252763=15r
1.252763/15 = r =0.083517531
Faster way to find x:
400/x e^r15 =1400/xe^r30
e^r30/e^r15 =1400/400 (the x's cancel)
e^r15 = 3.5
ln e^r15 = ln 3.5
r15 =1.252762968
r =1.252762968/15 =0.083517531

Now we can find initial amount x.
400= xe^(0.083517531)(15)
400 =x e^1.252763
e- is natural log = 2.718281828 , plug # in calculator and hit inverse ln works faster
e^1.252763 =3.5
400=x(3.5)
400/3.5 = x = 114.286 =P

The values for P and R never change.

What was the initial size? P =114.286 is initial amount

Find the doubling period in minutes. For doubling
Let left side (A/P) equal 2 and then find time.
2 = e^(0.083517531)t
Ln 2 =(0.083517531)t
0.69314718/0.083517531 = t = 8.3 min


Find the population after 115 minutes. Let t =115 and find A = 1,695,041

When (in minutes) will the population reach 15000? Let A =15000 and find t = 58.4 minutes
A T e^rt
400153.5
228.57148.292
1,00025.978.75
1,4003012.25
15,00058.39131.25
210,087.5901838.266
1,695,04111514831.61
5,932,64413051910.64
31,526,254150275854.7


What the three main and nastiest group of bacteria ruling earth presently?
Q. What are the main groups of bacteria ruling earth now?

There are three main groups of bacteria ruling earth from the Arabic world: The moon bacteria, the sun bacteria, and the stars bacteria, which call themselves 666.

As their ability is to mimic and replicate they have replaced the original worshipers of the sun and keep them enslaved. These Asian vermins have become white, black, yellow, and all species that they can replicate and so are the insects that are part of bacteria�s retinue.

The moon bacteria are worshipers of the moon. They live mostly in darkness or shade and the insects serving them are night insects and if they are there during the day, they keep the sun at a lower level of heat using the clouds.

The sun bacteria are not really from flame planet, but use the light that pierces the clouds to glorify themselves as the sun. In fact there is no bacteria that can resist flame.

The stars bacteria are the different branches of bacteria that are part or under the leadership of bacteria. They are replication of the main group. That is why when one kingdom does something, all other kingdoms follow.

These three main groups are the nastiest bacteria ruling earth. Bacteria keep surfacing the planet as the scientist bacteria are digging the earth to bring them out.

These bacteria are living entities that feed on decay. They lived in people before, those that were buried. They are waiting to be called to service because their hosts die. They take turn to rule. Those that live in king and kill their rulers have highest privileges. The privileges go by their faces on money and other things in the kingdom they rule. That is why only dead people are on money. As such, the vermins that ate these poor people made rich by bacteria are rewarded with their face on money, celebration of their prowess every year in the form of memorial and other nastiness that they did.

Their willing temples, the priests, and the rich, allowed them to do so as long as they continue to be in power.


Yah is a bacterial moon god. As all bacterial gods, they are bloodless and heartless. They are flies, ticks, lice, mites, roaches, and all types of seen and unseen bacteria. Bah, cah, tah, nah, and all the 26 letters of all alphabets in any languages are bacteria's names.

A. LIFE CONSUMES LIFE
~~~~~~~~~~~~
Life consumes Life
Take it for what it�s worth
Birth begs to be fed
Earth ate the dead
No matter what the spread
Iron Oxide rust red
�Life consumes Life� I said
Phage out numbers all of life combined
Bores into bacteria
Consumes until it dies
A Murder of crows to pick out the eyes
An eating frenzy never satisfied
Life is a banquet
A cannibalistic feast
For everything is the fodder
From the great whale to the hungered yeast


How much smaller is bacteria than dust or human hair strand that is the smallest human eye can see?
Q. Also is true that all types of bacteria cannot see ? If all types of bacteria cannot see how do they sense the world smell or chemicals?

The bacteria have no light rods or cones?

A. Bacteria are smaller than most eukaryotic cells, such as body cells. Viruses are so small they can infect bacteria.
Not bacteria can see. Some Protista have light sensitive patches to guide them to food, but they can't "see". Bacteria instead float around. They use chemicals to communicate with the world around them.





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Thursday, March 14, 2013

What the three main and nastiest group of bacteria ruling earth presently?

Q. What are the main groups of bacteria ruling earth now?

There are three main groups of bacteria ruling earth from the Arabic world: The moon bacteria, the sun bacteria, and the stars bacteria, which call themselves 666.

As their ability is to mimic and replicate they have replaced the original worshipers of the sun and keep them enslaved. These Asian vermins have become white, black, yellow, and all species that they can replicate and so are the insects that are part of bacteria�s retinue.

The moon bacteria are worshipers of the moon. They live mostly in darkness or shade and the insects serving them are night insects and if they are there during the day, they keep the sun at a lower level of heat using the clouds.

The sun bacteria are not really from flame planet, but use the light that pierces the clouds to glorify themselves as the sun. In fact there is no bacteria that can resist flame.

The stars bacteria are the different branches of bacteria that are part or under the leadership of bacteria. They are replication of the main group. That is why when one kingdom does something, all other kingdoms follow.

These three main groups are the nastiest bacteria ruling earth. Bacteria keep surfacing the planet as the scientist bacteria are digging the earth to bring them out.

These bacteria are living entities that feed on decay. They lived in people before, those that were buried. They are waiting to be called to service because their hosts die. They take turn to rule. Those that live in king and kill their rulers have highest privileges. The privileges go by their faces on money and other things in the kingdom they rule. That is why only dead people are on money. As such, the vermins that ate these poor people made rich by bacteria are rewarded with their face on money, celebration of their prowess every year in the form of memorial and other nastiness that they did.

Their willing temples, the priests, and the rich, allowed them to do so as long as they continue to be in power.


Yah is a bacterial moon god. As all bacterial gods, they are bloodless and heartless. They are flies, ticks, lice, mites, roaches, and all types of seen and unseen bacteria. Bah, cah, tah, nah, and all the 26 letters of all alphabets in any languages are bacteria's names.

A. LIFE CONSUMES LIFE
~~~~~~~~~~~~
Life consumes Life
Take it for what it�s worth
Birth begs to be fed
Earth ate the dead
No matter what the spread
Iron Oxide rust red
�Life consumes Life� I said
Phage out numbers all of life combined
Bores into bacteria
Consumes until it dies
A Murder of crows to pick out the eyes
An eating frenzy never satisfied
Life is a banquet
A cannibalistic feast
For everything is the fodder
From the great whale to the hungered yeast


How much smaller is bacteria than dust or human hair strand that is the smallest human eye can see?
Q. Also is true that all types of bacteria cannot see ? If all types of bacteria cannot see how do they sense the world smell or chemicals?

The bacteria have no light rods or cones?

A. Bacteria are smaller than most eukaryotic cells, such as body cells. Viruses are so small they can infect bacteria.
Not bacteria can see. Some Protista have light sensitive patches to guide them to food, but they can't "see". Bacteria instead float around. They use chemicals to communicate with the world around them.


What important roles do bacteria play in the deep ocean? What would happen if they werenʼt there?
Q. What important roles do bacteria play in the deep ocean? What would happen if
they werenʼt there?

A. Bacteria serve to rid the ocean floor of dead organisms by decomposing them. If they weren't there, the ocean floor would accumulate too much biomass.


How long does it take for good bacteria to form in a fish tank.And does the good bacteria break down the poop.?
Q. Its self.I know that the good bacteria feeds on ammonia but does it break down the poop it self.And how long does it take the good bacteria to form in the plastic case of the filter.I hear the term the filter media what does that mean.And how long can i go before i have to clean it.

A. Pooh will rot away causing amonia.. good bacteria will then eat the amonia converting it into nitrite..

A differnt type of bacteria needs to form eating the nitrite turning it into nitrate...

Nitrate is removed from he tank by frequent water changes (live plants and algae will also eat the nitrate)

The colonies of bacterias can take up to 6 months to form.. they form in the filter, gravel, and on any ornaments.. So it is best to only add a couple of hardy fish (zebra danios) for this period or read up on a fishless cycle..

Filter Media - is the sponglike inserts you put into a filter. There are variations on this depending on your filter.. After the intial 6 month period described above it is best to rinse the media in old tank water to maintain bacteria colony.. do this monthly..
And if it too blocked and need replacing only ever change half of the media at a time and ensure you rinse in old tank water

You use tank water you have poured into a seperate container because the chlorine is too high in our tap water and this will kill the fish.





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Sunday, March 3, 2013

What happens to the bacteria after extracting the insulin or hormone?

Q. I know that we use bacteria for DNA recombinant. After we extract whatever we need from the bacteria, what happens to the bacteria? I really want to know.

A. The bacteria are usually killed in the process.
After all, it's not as if you could milk them.

In some cases, the desired end product can be sseparated from the bacteria without killing them, such as skimming it off. In that case a continuous production is possible. Otherwise, you make it in batches.


The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Q. The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Find the doubling period in minutes
Find the population after 115 minutes
When (in minutes) will thle population reach 15000?
4 hours ago - 4 days left to answer.

A. Long answer...
A = Pe^rt
A -represents the amount after a certain amount of time
P- represents the the amount at the start
r -represents the rate or speed and is always represented as a decimal
t- represents the amount of time in years, months, days, hours , minutes
e- is natural log = 2.718281828
see: http://www.algebralab.org/Word/Word.aspx?file=Algebra_InterestIII.xml

1st we find the rate or speed of growth represented by letter r
A = xe^rt
400= xe^r15
400/x =e^r15
As rule: ln e^x =x
Ln(400/x) = ln e^r15= r15
Rule: ln(a/b) = ln a - lnb
Ln 400 �ln x =15r
Ln 400 -15r =lnx
5.99146-15r =lnx
Have to do the same for the 1400 bacteria.
1400= xe^r30
1400/x =e^r30
Ln(1400/x) = ln e^r30= r30
Ln 1400 �ln x =30r
Ln 1400 -30r =lnx
7.242275 -30r =lnx
Set the ln x�s to equal
7.242275 -30r = 5.99146-15r
1.252763=15r
1.252763/15 = r =0.083517531
Faster way to find x:
400/x e^r15 =1400/xe^r30
e^r30/e^r15 =1400/400 (the x's cancel)
e^r15 = 3.5
ln e^r15 = ln 3.5
r15 =1.252762968
r =1.252762968/15 =0.083517531

Now we can find initial amount x.
400= xe^(0.083517531)(15)
400 =x e^1.252763
e- is natural log = 2.718281828 , plug # in calculator and hit inverse ln works faster
e^1.252763 =3.5
400=x(3.5)
400/3.5 = x = 114.286 =P

The values for P and R never change.

What was the initial size? P =114.286 is initial amount

Find the doubling period in minutes. For doubling
Let left side (A/P) equal 2 and then find time.
2 = e^(0.083517531)t
Ln 2 =(0.083517531)t
0.69314718/0.083517531 = t = 8.3 min


Find the population after 115 minutes. Let t =115 and find A = 1,695,041

When (in minutes) will the population reach 15000? Let A =15000 and find t = 58.4 minutes
A T e^rt
400153.5
228.57148.292
1,00025.978.75
1,4003012.25
15,00058.39131.25
210,087.5901838.266
1,695,04111514831.61
5,932,64413051910.64
31,526,254150275854.7


What the three main and nastiest group of bacteria ruling earth presently?
Q. What are the main groups of bacteria ruling earth now?

There are three main groups of bacteria ruling earth from the Arabic world: The moon bacteria, the sun bacteria, and the stars bacteria, which call themselves 666.

As their ability is to mimic and replicate they have replaced the original worshipers of the sun and keep them enslaved. These Asian vermins have become white, black, yellow, and all species that they can replicate and so are the insects that are part of bacteria�s retinue.

The moon bacteria are worshipers of the moon. They live mostly in darkness or shade and the insects serving them are night insects and if they are there during the day, they keep the sun at a lower level of heat using the clouds.

The sun bacteria are not really from flame planet, but use the light that pierces the clouds to glorify themselves as the sun. In fact there is no bacteria that can resist flame.

The stars bacteria are the different branches of bacteria that are part or under the leadership of bacteria. They are replication of the main group. That is why when one kingdom does something, all other kingdoms follow.

These three main groups are the nastiest bacteria ruling earth. Bacteria keep surfacing the planet as the scientist bacteria are digging the earth to bring them out.

These bacteria are living entities that feed on decay. They lived in people before, those that were buried. They are waiting to be called to service because their hosts die. They take turn to rule. Those that live in king and kill their rulers have highest privileges. The privileges go by their faces on money and other things in the kingdom they rule. That is why only dead people are on money. As such, the vermins that ate these poor people made rich by bacteria are rewarded with their face on money, celebration of their prowess every year in the form of memorial and other nastiness that they did.

Their willing temples, the priests, and the rich, allowed them to do so as long as they continue to be in power.


Yah is a bacterial moon god. As all bacterial gods, they are bloodless and heartless. They are flies, ticks, lice, mites, roaches, and all types of seen and unseen bacteria. Bah, cah, tah, nah, and all the 26 letters of all alphabets in any languages are bacteria's names.

A. LIFE CONSUMES LIFE
~~~~~~~~~~~~
Life consumes Life
Take it for what it�s worth
Birth begs to be fed
Earth ate the dead
No matter what the spread
Iron Oxide rust red
�Life consumes Life� I said
Phage out numbers all of life combined
Bores into bacteria
Consumes until it dies
A Murder of crows to pick out the eyes
An eating frenzy never satisfied
Life is a banquet
A cannibalistic feast
For everything is the fodder
From the great whale to the hungered yeast


How much smaller is bacteria than dust or human hair strand that is the smallest human eye can see?
Q. Also is true that all types of bacteria cannot see ? If all types of bacteria cannot see how do they sense the world smell or chemicals?

The bacteria have no light rods or cones?

A. Bacteria are smaller than most eukaryotic cells, such as body cells. Viruses are so small they can infect bacteria.
Not bacteria can see. Some Protista have light sensitive patches to guide them to food, but they can't "see". Bacteria instead float around. They use chemicals to communicate with the world around them.





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Monday, February 11, 2013

What is the best method to know when bacteria is dying, or the best method to count bacteria in a petri dish?

Q. To clarify, if bacteria is in two petri dishes, and I add two different chemicals detrimental to the bacteria, what is the best way to tell which chemical is killing the bacteria fastest? Thanks!

A. I would suggest a method similar to that used in testing antimicrobial susceptibility. (1) Impregnate an known similar concetration of your chemical each on its own paper disk. (2) spreas your bacteria evenly on appropriate medium. (3) stamp the chemical disks on top of medium and incubate at 37 degrees for a day. (4) measure the zone of inhibition (area surrounding chemical disk with no bacterial growth). The larger the area, the more effective the chemical.
N.B. there may also be issues of chemical diffusion etc.


What happens to the bacteria after extracting the insulin or hormone?
Q. I know that we use bacteria for DNA recombinant. After we extract whatever we need from the bacteria, what happens to the bacteria? I really want to know.

A. The bacteria are usually killed in the process.
After all, it's not as if you could milk them.

In some cases, the desired end product can be sseparated from the bacteria without killing them, such as skimming it off. In that case a continuous production is possible. Otherwise, you make it in batches.


The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Q. The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Find the doubling period in minutes
Find the population after 115 minutes
When (in minutes) will thle population reach 15000?
4 hours ago - 4 days left to answer.

A. Long answer...
A = Pe^rt
A -represents the amount after a certain amount of time
P- represents the the amount at the start
r -represents the rate or speed and is always represented as a decimal
t- represents the amount of time in years, months, days, hours , minutes
e- is natural log = 2.718281828
see: http://www.algebralab.org/Word/Word.aspx?file=Algebra_InterestIII.xml

1st we find the rate or speed of growth represented by letter r
A = xe^rt
400= xe^r15
400/x =e^r15
As rule: ln e^x =x
Ln(400/x) = ln e^r15= r15
Rule: ln(a/b) = ln a - lnb
Ln 400 �ln x =15r
Ln 400 -15r =lnx
5.99146-15r =lnx
Have to do the same for the 1400 bacteria.
1400= xe^r30
1400/x =e^r30
Ln(1400/x) = ln e^r30= r30
Ln 1400 �ln x =30r
Ln 1400 -30r =lnx
7.242275 -30r =lnx
Set the ln x�s to equal
7.242275 -30r = 5.99146-15r
1.252763=15r
1.252763/15 = r =0.083517531
Faster way to find x:
400/x e^r15 =1400/xe^r30
e^r30/e^r15 =1400/400 (the x's cancel)
e^r15 = 3.5
ln e^r15 = ln 3.5
r15 =1.252762968
r =1.252762968/15 =0.083517531

Now we can find initial amount x.
400= xe^(0.083517531)(15)
400 =x e^1.252763
e- is natural log = 2.718281828 , plug # in calculator and hit inverse ln works faster
e^1.252763 =3.5
400=x(3.5)
400/3.5 = x = 114.286 =P

The values for P and R never change.

What was the initial size? P =114.286 is initial amount

Find the doubling period in minutes. For doubling
Let left side (A/P) equal 2 and then find time.
2 = e^(0.083517531)t
Ln 2 =(0.083517531)t
0.69314718/0.083517531 = t = 8.3 min


Find the population after 115 minutes. Let t =115 and find A = 1,695,041

When (in minutes) will the population reach 15000? Let A =15000 and find t = 58.4 minutes
A T e^rt
400153.5
228.57148.292
1,00025.978.75
1,4003012.25
15,00058.39131.25
210,087.5901838.266
1,695,04111514831.61
5,932,64413051910.64
31,526,254150275854.7


What the three main and nastiest group of bacteria ruling earth presently?
Q. What are the main groups of bacteria ruling earth now?

There are three main groups of bacteria ruling earth from the Arabic world: The moon bacteria, the sun bacteria, and the stars bacteria, which call themselves 666.

As their ability is to mimic and replicate they have replaced the original worshipers of the sun and keep them enslaved. These Asian vermins have become white, black, yellow, and all species that they can replicate and so are the insects that are part of bacteria�s retinue.

The moon bacteria are worshipers of the moon. They live mostly in darkness or shade and the insects serving them are night insects and if they are there during the day, they keep the sun at a lower level of heat using the clouds.

The sun bacteria are not really from flame planet, but use the light that pierces the clouds to glorify themselves as the sun. In fact there is no bacteria that can resist flame.

The stars bacteria are the different branches of bacteria that are part or under the leadership of bacteria. They are replication of the main group. That is why when one kingdom does something, all other kingdoms follow.

These three main groups are the nastiest bacteria ruling earth. Bacteria keep surfacing the planet as the scientist bacteria are digging the earth to bring them out.

These bacteria are living entities that feed on decay. They lived in people before, those that were buried. They are waiting to be called to service because their hosts die. They take turn to rule. Those that live in king and kill their rulers have highest privileges. The privileges go by their faces on money and other things in the kingdom they rule. That is why only dead people are on money. As such, the vermins that ate these poor people made rich by bacteria are rewarded with their face on money, celebration of their prowess every year in the form of memorial and other nastiness that they did.

Their willing temples, the priests, and the rich, allowed them to do so as long as they continue to be in power.


Yah is a bacterial moon god. As all bacterial gods, they are bloodless and heartless. They are flies, ticks, lice, mites, roaches, and all types of seen and unseen bacteria. Bah, cah, tah, nah, and all the 26 letters of all alphabets in any languages are bacteria's names.

A. LIFE CONSUMES LIFE
~~~~~~~~~~~~
Life consumes Life
Take it for what it�s worth
Birth begs to be fed
Earth ate the dead
No matter what the spread
Iron Oxide rust red
�Life consumes Life� I said
Phage out numbers all of life combined
Bores into bacteria
Consumes until it dies
A Murder of crows to pick out the eyes
An eating frenzy never satisfied
Life is a banquet
A cannibalistic feast
For everything is the fodder
From the great whale to the hungered yeast





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Saturday, February 9, 2013

How much smaller is bacteria than dust or human hair strand that is the smallest human eye can see?

Q. Also is true that all types of bacteria cannot see ? If all types of bacteria cannot see how do they sense the world smell or chemicals?

The bacteria have no light rods or cones?

A. Bacteria are smaller than most eukaryotic cells, such as body cells. Viruses are so small they can infect bacteria.
Not bacteria can see. Some Protista have light sensitive patches to guide them to food, but they can't "see". Bacteria instead float around. They use chemicals to communicate with the world around them.


What important roles do bacteria play in the deep ocean? What would happen if they werenʼt there?
Q. What important roles do bacteria play in the deep ocean? What would happen if
they werenʼt there?

A. Bacteria serve to rid the ocean floor of dead organisms by decomposing them. If they weren't there, the ocean floor would accumulate too much biomass.


How long does it take for good bacteria to form in a fish tank.And does the good bacteria break down the poop.?
Q. Its self.I know that the good bacteria feeds on ammonia but does it break down the poop it self.And how long does it take the good bacteria to form in the plastic case of the filter.I hear the term the filter media what does that mean.And how long can i go before i have to clean it.

A. Pooh will rot away causing amonia.. good bacteria will then eat the amonia converting it into nitrite..

A differnt type of bacteria needs to form eating the nitrite turning it into nitrate...

Nitrate is removed from he tank by frequent water changes (live plants and algae will also eat the nitrate)

The colonies of bacterias can take up to 6 months to form.. they form in the filter, gravel, and on any ornaments.. So it is best to only add a couple of hardy fish (zebra danios) for this period or read up on a fishless cycle..

Filter Media - is the sponglike inserts you put into a filter. There are variations on this depending on your filter.. After the intial 6 month period described above it is best to rinse the media in old tank water to maintain bacteria colony.. do this monthly..
And if it too blocked and need replacing only ever change half of the media at a time and ensure you rinse in old tank water

You use tank water you have poured into a seperate container because the chlorine is too high in our tap water and this will kill the fish.


What temperature is good bacteria killed?
Q. I always hear bad bacteria is killed at 145 degrees for a certain amount of time, but what about good bacteria. Is it killed in the same range or at higher temperatures? I hear that low temperature pasterization of milk kills off the bad bacteria and preserves some of the good bacteria and it's heated at 145 degrees for 30 minutes. Is this true or are they giving false statements to sell this milk?

A. Unless the bacterium has an endospore which is resistant to heat and chemicals, the thermal death time is the same for most species of bacteria, good or bad. Pasteurization of milk is intended to kill pathogenic bacteria typically found in milk. The standards for the quality of milk is set by state and federal agencies. Those bacteria found in pasteurized milk are considered thermoduric (tolerate the pasteurization temperature) and psychrophilic (can grow at refrigeration temperatures) and will eventually cause the milk to spoil. With ultra-pasteurization, milk does not have to be refrigerated.





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Monday, February 4, 2013

How much smaller is bacteria than dust or human hair strand that is the smallest human eye can see?

Q. Also is true that all types of bacteria cannot see ? If all types of bacteria cannot see how do they sense the world smell or chemicals?

The bacteria have no light rods or cones?

A. Bacteria are smaller than most eukaryotic cells, such as body cells. Viruses are so small they can infect bacteria.
Not bacteria can see. Some Protista have light sensitive patches to guide them to food, but they can't "see". Bacteria instead float around. They use chemicals to communicate with the world around them.


What important roles do bacteria play in the deep ocean? What would happen if they werenʼt there?
Q. What important roles do bacteria play in the deep ocean? What would happen if
they werenʼt there?

A. Bacteria serve to rid the ocean floor of dead organisms by decomposing them. If they weren't there, the ocean floor would accumulate too much biomass.


How long does it take for good bacteria to form in a fish tank.And does the good bacteria break down the poop.?
Q. Its self.I know that the good bacteria feeds on ammonia but does it break down the poop it self.And how long does it take the good bacteria to form in the plastic case of the filter.I hear the term the filter media what does that mean.And how long can i go before i have to clean it.

A. Pooh will rot away causing amonia.. good bacteria will then eat the amonia converting it into nitrite..

A differnt type of bacteria needs to form eating the nitrite turning it into nitrate...

Nitrate is removed from he tank by frequent water changes (live plants and algae will also eat the nitrate)

The colonies of bacterias can take up to 6 months to form.. they form in the filter, gravel, and on any ornaments.. So it is best to only add a couple of hardy fish (zebra danios) for this period or read up on a fishless cycle..

Filter Media - is the sponglike inserts you put into a filter. There are variations on this depending on your filter.. After the intial 6 month period described above it is best to rinse the media in old tank water to maintain bacteria colony.. do this monthly..
And if it too blocked and need replacing only ever change half of the media at a time and ensure you rinse in old tank water

You use tank water you have poured into a seperate container because the chlorine is too high in our tap water and this will kill the fish.


What temperature is good bacteria killed?
Q. I always hear bad bacteria is killed at 145 degrees for a certain amount of time, but what about good bacteria. Is it killed in the same range or at higher temperatures? I hear that low temperature pasterization of milk kills off the bad bacteria and preserves some of the good bacteria and it's heated at 145 degrees for 30 minutes. Is this true or are they giving false statements to sell this milk?

A. Unless the bacterium has an endospore which is resistant to heat and chemicals, the thermal death time is the same for most species of bacteria, good or bad. Pasteurization of milk is intended to kill pathogenic bacteria typically found in milk. The standards for the quality of milk is set by state and federal agencies. Those bacteria found in pasteurized milk are considered thermoduric (tolerate the pasteurization temperature) and psychrophilic (can grow at refrigeration temperatures) and will eventually cause the milk to spoil. With ultra-pasteurization, milk does not have to be refrigerated.





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Friday, January 25, 2013

What the three main and nastiest group of bacteria ruling earth presently?

Q. What are the main groups of bacteria ruling earth now?

There are three main groups of bacteria ruling earth from the Arabic world: The moon bacteria, the sun bacteria, and the stars bacteria, which call themselves 666.

As their ability is to mimic and replicate they have replaced the original worshipers of the sun and keep them enslaved. These Asian vermins have become white, black, yellow, and all species that they can replicate and so are the insects that are part of bacteria�s retinue.

The moon bacteria are worshipers of the moon. They live mostly in darkness or shade and the insects serving them are night insects and if they are there during the day, they keep the sun at a lower level of heat using the clouds.

The sun bacteria are not really from flame planet, but use the light that pierces the clouds to glorify themselves as the sun. In fact there is no bacteria that can resist flame.

The stars bacteria are the different branches of bacteria that are part or under the leadership of bacteria. They are replication of the main group. That is why when one kingdom does something, all other kingdoms follow.

These three main groups are the nastiest bacteria ruling earth. Bacteria keep surfacing the planet as the scientist bacteria are digging the earth to bring them out.

These bacteria are living entities that feed on decay. They lived in people before, those that were buried. They are waiting to be called to service because their hosts die. They take turn to rule. Those that live in king and kill their rulers have highest privileges. The privileges go by their faces on money and other things in the kingdom they rule. That is why only dead people are on money. As such, the vermins that ate these poor people made rich by bacteria are rewarded with their face on money, celebration of their prowess every year in the form of memorial and other nastiness that they did.

Their willing temples, the priests, and the rich, allowed them to do so as long as they continue to be in power.


Yah is a bacterial moon god. As all bacterial gods, they are bloodless and heartless. They are flies, ticks, lice, mites, roaches, and all types of seen and unseen bacteria. Bah, cah, tah, nah, and all the 26 letters of all alphabets in any languages are bacteria's names.

A. LIFE CONSUMES LIFE
~~~~~~~~~~~~
Life consumes Life
Take it for what it�s worth
Birth begs to be fed
Earth ate the dead
No matter what the spread
Iron Oxide rust red
�Life consumes Life� I said
Phage out numbers all of life combined
Bores into bacteria
Consumes until it dies
A Murder of crows to pick out the eyes
An eating frenzy never satisfied
Life is a banquet
A cannibalistic feast
For everything is the fodder
From the great whale to the hungered yeast


How much smaller is bacteria than dust or human hair strand that is the smallest human eye can see?
Q. Also is true that all types of bacteria cannot see ? If all types of bacteria cannot see how do they sense the world smell or chemicals?

The bacteria have no light rods or cones?

A. Bacteria are smaller than most eukaryotic cells, such as body cells. Viruses are so small they can infect bacteria.
Not bacteria can see. Some Protista have light sensitive patches to guide them to food, but they can't "see". Bacteria instead float around. They use chemicals to communicate with the world around them.


What important roles do bacteria play in the deep ocean? What would happen if they werenʼt there?
Q. What important roles do bacteria play in the deep ocean? What would happen if
they werenʼt there?

A. Bacteria serve to rid the ocean floor of dead organisms by decomposing them. If they weren't there, the ocean floor would accumulate too much biomass.


How long does it take for good bacteria to form in a fish tank.And does the good bacteria break down the poop.?
Q. Its self.I know that the good bacteria feeds on ammonia but does it break down the poop it self.And how long does it take the good bacteria to form in the plastic case of the filter.I hear the term the filter media what does that mean.And how long can i go before i have to clean it.

A. Pooh will rot away causing amonia.. good bacteria will then eat the amonia converting it into nitrite..

A differnt type of bacteria needs to form eating the nitrite turning it into nitrate...

Nitrate is removed from he tank by frequent water changes (live plants and algae will also eat the nitrate)

The colonies of bacterias can take up to 6 months to form.. they form in the filter, gravel, and on any ornaments.. So it is best to only add a couple of hardy fish (zebra danios) for this period or read up on a fishless cycle..

Filter Media - is the sponglike inserts you put into a filter. There are variations on this depending on your filter.. After the intial 6 month period described above it is best to rinse the media in old tank water to maintain bacteria colony.. do this monthly..
And if it too blocked and need replacing only ever change half of the media at a time and ensure you rinse in old tank water

You use tank water you have poured into a seperate container because the chlorine is too high in our tap water and this will kill the fish.





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Thursday, January 24, 2013

The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?

Q. The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Find the doubling period in minutes
Find the population after 115 minutes
When (in minutes) will thle population reach 15000?
4 hours ago - 4 days left to answer.

A. Long answer...
A = Pe^rt
A -represents the amount after a certain amount of time
P- represents the the amount at the start
r -represents the rate or speed and is always represented as a decimal
t- represents the amount of time in years, months, days, hours , minutes
e- is natural log = 2.718281828
see: http://www.algebralab.org/Word/Word.aspx?file=Algebra_InterestIII.xml

1st we find the rate or speed of growth represented by letter r
A = xe^rt
400= xe^r15
400/x =e^r15
As rule: ln e^x =x
Ln(400/x) = ln e^r15= r15
Rule: ln(a/b) = ln a - lnb
Ln 400 �ln x =15r
Ln 400 -15r =lnx
5.99146-15r =lnx
Have to do the same for the 1400 bacteria.
1400= xe^r30
1400/x =e^r30
Ln(1400/x) = ln e^r30= r30
Ln 1400 �ln x =30r
Ln 1400 -30r =lnx
7.242275 -30r =lnx
Set the ln x�s to equal
7.242275 -30r = 5.99146-15r
1.252763=15r
1.252763/15 = r =0.083517531
Faster way to find x:
400/x e^r15 =1400/xe^r30
e^r30/e^r15 =1400/400 (the x's cancel)
e^r15 = 3.5
ln e^r15 = ln 3.5
r15 =1.252762968
r =1.252762968/15 =0.083517531

Now we can find initial amount x.
400= xe^(0.083517531)(15)
400 =x e^1.252763
e- is natural log = 2.718281828 , plug # in calculator and hit inverse ln works faster
e^1.252763 =3.5
400=x(3.5)
400/3.5 = x = 114.286 =P

The values for P and R never change.

What was the initial size? P =114.286 is initial amount

Find the doubling period in minutes. For doubling
Let left side (A/P) equal 2 and then find time.
2 = e^(0.083517531)t
Ln 2 =(0.083517531)t
0.69314718/0.083517531 = t = 8.3 min


Find the population after 115 minutes. Let t =115 and find A = 1,695,041

When (in minutes) will the population reach 15000? Let A =15000 and find t = 58.4 minutes
A T e^rt
400153.5
228.57148.292
1,00025.978.75
1,4003012.25
15,00058.39131.25
210,087.5901838.266
1,695,04111514831.61
5,932,64413051910.64
31,526,254150275854.7


What the three main and nastiest group of bacteria ruling earth presently?
Q. What are the main groups of bacteria ruling earth now?

There are three main groups of bacteria ruling earth from the Arabic world: The moon bacteria, the sun bacteria, and the stars bacteria, which call themselves 666.

As their ability is to mimic and replicate they have replaced the original worshipers of the sun and keep them enslaved. These Asian vermins have become white, black, yellow, and all species that they can replicate and so are the insects that are part of bacteria�s retinue.

The moon bacteria are worshipers of the moon. They live mostly in darkness or shade and the insects serving them are night insects and if they are there during the day, they keep the sun at a lower level of heat using the clouds.

The sun bacteria are not really from flame planet, but use the light that pierces the clouds to glorify themselves as the sun. In fact there is no bacteria that can resist flame.

The stars bacteria are the different branches of bacteria that are part or under the leadership of bacteria. They are replication of the main group. That is why when one kingdom does something, all other kingdoms follow.

These three main groups are the nastiest bacteria ruling earth. Bacteria keep surfacing the planet as the scientist bacteria are digging the earth to bring them out.

These bacteria are living entities that feed on decay. They lived in people before, those that were buried. They are waiting to be called to service because their hosts die. They take turn to rule. Those that live in king and kill their rulers have highest privileges. The privileges go by their faces on money and other things in the kingdom they rule. That is why only dead people are on money. As such, the vermins that ate these poor people made rich by bacteria are rewarded with their face on money, celebration of their prowess every year in the form of memorial and other nastiness that they did.

Their willing temples, the priests, and the rich, allowed them to do so as long as they continue to be in power.


Yah is a bacterial moon god. As all bacterial gods, they are bloodless and heartless. They are flies, ticks, lice, mites, roaches, and all types of seen and unseen bacteria. Bah, cah, tah, nah, and all the 26 letters of all alphabets in any languages are bacteria's names.

A. LIFE CONSUMES LIFE
~~~~~~~~~~~~
Life consumes Life
Take it for what it�s worth
Birth begs to be fed
Earth ate the dead
No matter what the spread
Iron Oxide rust red
�Life consumes Life� I said
Phage out numbers all of life combined
Bores into bacteria
Consumes until it dies
A Murder of crows to pick out the eyes
An eating frenzy never satisfied
Life is a banquet
A cannibalistic feast
For everything is the fodder
From the great whale to the hungered yeast


How much smaller is bacteria than dust or human hair strand that is the smallest human eye can see?
Q. Also is true that all types of bacteria cannot see ? If all types of bacteria cannot see how do they sense the world smell or chemicals?

The bacteria have no light rods or cones?

A. Bacteria are smaller than most eukaryotic cells, such as body cells. Viruses are so small they can infect bacteria.
Not bacteria can see. Some Protista have light sensitive patches to guide them to food, but they can't "see". Bacteria instead float around. They use chemicals to communicate with the world around them.


How long does it take for good bacteria to form in a fish tank.And does the good bacteria break down the poop.?
Q. Its self.I know that the good bacteria feeds on ammonia but does it break down the poop it self.And how long does it take the good bacteria to form in the plastic case of the filter.I hear the term the filter media what does that mean.And how long can i go before i have to clean it.

A. Pooh will rot away causing amonia.. good bacteria will then eat the amonia converting it into nitrite..

A differnt type of bacteria needs to form eating the nitrite turning it into nitrate...

Nitrate is removed from he tank by frequent water changes (live plants and algae will also eat the nitrate)

The colonies of bacterias can take up to 6 months to form.. they form in the filter, gravel, and on any ornaments.. So it is best to only add a couple of hardy fish (zebra danios) for this period or read up on a fishless cycle..

Filter Media - is the sponglike inserts you put into a filter. There are variations on this depending on your filter.. After the intial 6 month period described above it is best to rinse the media in old tank water to maintain bacteria colony.. do this monthly..
And if it too blocked and need replacing only ever change half of the media at a time and ensure you rinse in old tank water

You use tank water you have poured into a seperate container because the chlorine is too high in our tap water and this will kill the fish.





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