Sunday, January 6, 2013

What happens to the bacteria after extracting the insulin or hormone?

Q. I know that we use bacteria for DNA recombinant. After we extract whatever we need from the bacteria, what happens to the bacteria? I really want to know.

A. The bacteria are usually killed in the process.
After all, it's not as if you could milk them.

In some cases, the desired end product can be sseparated from the bacteria without killing them, such as skimming it off. In that case a continuous production is possible. Otherwise, you make it in batches.


The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Q. The count in a bacteria culture was 400 after 15 minutes and 1400 after 30 minutes. What was the initial siz?
Find the doubling period in minutes
Find the population after 115 minutes
When (in minutes) will thle population reach 15000?
4 hours ago - 4 days left to answer.

A. Long answer...
A = Pe^rt
A -represents the amount after a certain amount of time
P- represents the the amount at the start
r -represents the rate or speed and is always represented as a decimal
t- represents the amount of time in years, months, days, hours , minutes
e- is natural log = 2.718281828
see: http://www.algebralab.org/Word/Word.aspx?file=Algebra_InterestIII.xml

1st we find the rate or speed of growth represented by letter r
A = xe^rt
400= xe^r15
400/x =e^r15
As rule: ln e^x =x
Ln(400/x) = ln e^r15= r15
Rule: ln(a/b) = ln a - lnb
Ln 400 �ln x =15r
Ln 400 -15r =lnx
5.99146-15r =lnx
Have to do the same for the 1400 bacteria.
1400= xe^r30
1400/x =e^r30
Ln(1400/x) = ln e^r30= r30
Ln 1400 �ln x =30r
Ln 1400 -30r =lnx
7.242275 -30r =lnx
Set the ln x�s to equal
7.242275 -30r = 5.99146-15r
1.252763=15r
1.252763/15 = r =0.083517531
Faster way to find x:
400/x e^r15 =1400/xe^r30
e^r30/e^r15 =1400/400 (the x's cancel)
e^r15 = 3.5
ln e^r15 = ln 3.5
r15 =1.252762968
r =1.252762968/15 =0.083517531

Now we can find initial amount x.
400= xe^(0.083517531)(15)
400 =x e^1.252763
e- is natural log = 2.718281828 , plug # in calculator and hit inverse ln works faster
e^1.252763 =3.5
400=x(3.5)
400/3.5 = x = 114.286 =P

The values for P and R never change.

What was the initial size? P =114.286 is initial amount

Find the doubling period in minutes. For doubling
Let left side (A/P) equal 2 and then find time.
2 = e^(0.083517531)t
Ln 2 =(0.083517531)t
0.69314718/0.083517531 = t = 8.3 min


Find the population after 115 minutes. Let t =115 and find A = 1,695,041

When (in minutes) will the population reach 15000? Let A =15000 and find t = 58.4 minutes
A T e^rt
400153.5
228.57148.292
1,00025.978.75
1,4003012.25
15,00058.39131.25
210,087.5901838.266
1,695,04111514831.61
5,932,64413051910.64
31,526,254150275854.7


What the three main and nastiest group of bacteria ruling earth presently?
Q. What are the main groups of bacteria ruling earth now?

There are three main groups of bacteria ruling earth from the Arabic world: The moon bacteria, the sun bacteria, and the stars bacteria, which call themselves 666.

As their ability is to mimic and replicate they have replaced the original worshipers of the sun and keep them enslaved. These Asian vermins have become white, black, yellow, and all species that they can replicate and so are the insects that are part of bacteria�s retinue.

The moon bacteria are worshipers of the moon. They live mostly in darkness or shade and the insects serving them are night insects and if they are there during the day, they keep the sun at a lower level of heat using the clouds.

The sun bacteria are not really from flame planet, but use the light that pierces the clouds to glorify themselves as the sun. In fact there is no bacteria that can resist flame.

The stars bacteria are the different branches of bacteria that are part or under the leadership of bacteria. They are replication of the main group. That is why when one kingdom does something, all other kingdoms follow.

These three main groups are the nastiest bacteria ruling earth. Bacteria keep surfacing the planet as the scientist bacteria are digging the earth to bring them out.

These bacteria are living entities that feed on decay. They lived in people before, those that were buried. They are waiting to be called to service because their hosts die. They take turn to rule. Those that live in king and kill their rulers have highest privileges. The privileges go by their faces on money and other things in the kingdom they rule. That is why only dead people are on money. As such, the vermins that ate these poor people made rich by bacteria are rewarded with their face on money, celebration of their prowess every year in the form of memorial and other nastiness that they did.

Their willing temples, the priests, and the rich, allowed them to do so as long as they continue to be in power.


Yah is a bacterial moon god. As all bacterial gods, they are bloodless and heartless. They are flies, ticks, lice, mites, roaches, and all types of seen and unseen bacteria. Bah, cah, tah, nah, and all the 26 letters of all alphabets in any languages are bacteria's names.

A. LIFE CONSUMES LIFE
~~~~~~~~~~~~
Life consumes Life
Take it for what it�s worth
Birth begs to be fed
Earth ate the dead
No matter what the spread
Iron Oxide rust red
�Life consumes Life� I said
Phage out numbers all of life combined
Bores into bacteria
Consumes until it dies
A Murder of crows to pick out the eyes
An eating frenzy never satisfied
Life is a banquet
A cannibalistic feast
For everything is the fodder
From the great whale to the hungered yeast


How much smaller is bacteria than dust or human hair strand that is the smallest human eye can see?
Q. Also is true that all types of bacteria cannot see ? If all types of bacteria cannot see how do they sense the world smell or chemicals?

The bacteria have no light rods or cones?

A. Bacteria are smaller than most eukaryotic cells, such as body cells. Viruses are so small they can infect bacteria.
Not bacteria can see. Some Protista have light sensitive patches to guide them to food, but they can't "see". Bacteria instead float around. They use chemicals to communicate with the world around them.





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